The Today Show's Karl Stefanovic and Ally Langdon were unable to contain themselves on Friday morning while listening to a snippet of Meghan Markle reading her children's book The Bench aloud on YouTube.
News and sports presenter Alex Cullen was also unable to keep a straight face while listening to the Duchess of Sussex read the book and dedicate it to Prince Harry and their son Archie.
"For the man and the boy who make my heart go pump-pump," Meghan read seriously in the clip.
Alex quickly broke out in laughter, saying, "Sorry! It's very hard to keep a straight face!"
"Why is it difficult to keep a straight face, Alex? It's a lovely story," Ally teased him.
"As we always say, pump-pump," Karl quipped, causing Ally to laugh and sing lyrics from the 1989 song 'Pump Up The Jam', saying, "Up the jam!"
However, some Twitter users were unimpressed by the sledges with one user writing, "What is Karl Stefanovic's problem. His hatred of Meghan is absolutely ridiculous and his behaviour on the air is bullying and unprofessional."
"He used to be a decent presenter once. Now his ego is pathetic. Why his offsider belittles herself by laughing at Meghan is beyond me," another person said.
Someone else compared Karl to Piers Morgan, who is known for his commentary of the royal couple.
"Karl is pretty much mini Piers on anything Meghan or Harry," they wrote.
Karl and Ally have previously mocked Meghan and her book, after finding out it stemmed from a poem, with the pair calling it ridiculous that the Sussexes might write each other poetry in their spare time.
During the segment in May, Ally questioned the words on the autocue, comparing Meghan to famous children's authors.
"Meghan Markle is joining the ranks of Roald Dahl and J.K. Rowling - oh, a stretch - by writing her first children's book about the bond between fathers and sons," she said.
"Yeah, can you imagine anything better? I mean, pop it on in the car, pop it on at home when you're cooking dinner. Meghan's dulcet tones," Nine's US correspondent Amelia Adams added.
Karl slammed his desk, bursting into laughter saying, "They actually write poetry together!"
At the time one Twitter user shared that they didn't appreciate his response and wrote, "[Karl], I saw a clip of your reaction... rude and unnecessary. Don't buy the book if you don't like it, it's that simple. No need to mock."
Never miss a thing. Sign up to Yahoo Lifestyle’s daily newsletter.
Or if you have a story idea, email us at email@example.com.