The Project's Kate Langbroek left her co-hosts momentarily stunned when she made a rude joke about Peter Helliar on Thursday night.
The hosts were talking about Waleed Aly, who received his Covid-19 vaccine with the 42-year-old saying he didn't feel any pain.
The presenter said it was "almost underwhelming because I felt almost nothing."
"I don't think there was any pain at all. I feel completely normal now," he added.
"Well, you know what? You work with Pete Helliar every night, you're used to a little prick!" Kate quipped.
After a moment, Pete laughed, adding: "She's back! You had two seconds off!"
He later jokingly clarified: "I'm actually a big prick to be honest!"
Waleed tried to steer the conversation back to the vaccine, saying it was "a really important message" for others to go out and get vaccinated, especially as many ethnic groups have been apprehensive about getting the jab.
He added that there was "nothing to be scared of" and that "you're really doing a great service for yourselves and your community".
Kate told viewers to check that he's on the show every night for the next week, as some people have been known to feel slight side effects from the vaccine.
Waleed then joked, "If Kate doesn't have any more dirty puns we'll throw to a break."
It comes after Carrie Bickmore slammed the vaccine rollout, which has seen aged care residents waiting for a jab.
"This makes me so angry,” Carrie said.
"We've got family in aged care as well waiting for their second doses and it's taking far too long.
"Especially given what we saw happen last year. We know what happens if you don’t look after the elderly."
Of Australia's 910 coronavirus deaths, 685 have been aged care residents.
More than 4.2 million vaccine doses have been administered, a target the government initially set for March.
Never miss a thing. Sign up to Yahoo Lifestyle’s daily newsletter.
Or if you have a story idea, email us at firstname.lastname@example.org.