The Queen could be seen watching the traditional flypast with Prince Charles, his wife Camilla, Prince William, Kate Middleton, and their children Prince George, Princess Charlotte and Prince Louis.
While the royal family were all smiles, fans pointed out how few family members appeared compared to past years, with Prince Harry, Meghan Markle and Prince Andrew among those omitted from the balcony.
Only working members of the royal family were invited to watch the flypast from the balcony this year, with the exception of Prince William and Kate's children and Princess Anne's husband.
In 2019 prior to the pandemic, there were about 40 royals present on the balcony during the Trooping the Colour event, a far cry from the 18 who appeared this year.
"So empty. A family must welcome everyone despite thoughts or feelings..." one commented on Instagram.
"What a shame the balcony is so sparse," another added.
"So incomplete without Prince Philip and Prince Harry," a third said.
Many questioned why Meghan and Harry were not on the balcony, while others suggested the gap left between the Queen and Kate was a subtle tribute to Prince Philip, who died in April last year.
"She left a space for Prince Philip next to her," one commented.
"I thought that too!" another responded.
"I saw that immediately and broke down in tears," a fan said.
Queen pulls out of major event
Buckingham Palace has announced that the Queen has pulled out of a major event due to “discomfort”.
Her Majesty won’t be attending the Service of Thanksgiving, which is taking place at St Paul’s Cathedral on Friday. The palace said the decision to pull out was made with “great reluctance” and blamed the Queen’s ongoing mobility issues, after considering the “journey and activity required” for the event.
The announcement came just hours after Her Majesty appeared on the Palace balcony for Trooping the Colour, where she was seen using a walking stick. While she appeared to enjoy the ceremony, the Palace alluded to her mobility issues in their statement.
“The Queen greatly enjoyed today’s Birthday Parade and Flypast but did experience some discomfort,” they began.
“Taking into account the journey and activity required to participate in tomorrow’s National Service of Thanksgiving at St Paul’s Cathedral, Her Majesty with great reluctance has concluded that she will not attend.”
There have been health concerns for the monarch recently after she missed a string of events over Easter and announced she would no longer be attending any of her garden parties.
Never miss a thing. Sign up to Yahoo Lifestyle’s daily newsletter.
Or if you have a story idea, email us at email@example.com.