It comes after his co-host Jackie 'O' Henderson has been forced to continually deny she has used the drug to lose 18kg last year.
While speaking about Gwen Stefani, Kyle, Jackie and newsreader Brooklyn Ross speculated that the No Doubt singer had undergone surgery to continue looking incredible in her 50s.
"I wonder if she's had surgery? I bet she has," Jackie questioned, before Kyle hit back, saying, "Imagine if people are talking about you like that!"
"They do, Kyle!" Jackie said. "They're always speculating!"
"You look good, but you don't look good enough to have had surgery," Kyle quipped.
Brooklyn then referenced the Ozempic rumours, saying that he was speaking with his mum, who told him, "Kyle – he's got to be on Ozempic!"
"Me? What have I done?" Kyle questioned, surprised as Jackie laughed, telling him it was his turn to be hit with the rumour.
"You're losing weight, my mum thinks you're on Ozempic!" Brooklyn said.
Jackie questioned whether Brooklyn's mum also thought she was on Ozempic, despite her constant attempts to shut the rumour down, with the newsreader telling her that she does think that.
"I can't convince anyone, can I?" Jackie said. "Like I don't care, but I just wish that people would know that if I was I'd just say it at this point."
However, she added that she wouldn't have said it if she was still in partnership with WW (Weight Watchers), "I wouldn't have, like I'm going to be honest and say I couldn't do that. But there's nothing stopping me from saying it and I would say it, but I can't convince people otherwise."
Kyle added that he believed that Brooklyn's mum had just seen a "good angle" of him, saying, "Maybe I've stopped [consuming] as much bread and Coca-Cola."
"I tell you what, you always come back from the break a little bit skinnier," Jackie said. "Because you've been healthy out by the sun, you're less into like the 12 coffees."
He joked that his wife has been giving him salads instead of ice cream and he's been eating it anyway because it's in front of him.
"You do look good," Jackie told him. "Keep it going, get on the Ozempic!"
"I don't care about being some thin flop!" Kyle hit back, with Jackie and Brooklyn telling him it would help his health issues if he continued to lose weight.
The host concluded by saying he wouldn't be taking medical advice from Jackie and Brooklyn.
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