Kanye West dissed the 32-year-old Sexy Back singer at his concert in February, and now Justin Timberlake has fired back.
Timberlake performed his new single Suit & Tie alongside Jay-Z on Saturday Night Live on March 9, and changed the lyrics of the song to slam West, 35.
In the song's original lyrics, Timberlake sings:
"Aww, sh-t so sick, got a hit and picked up a habit."
However, for his SNL performance, the singer crooned:
"My hit's so sick, got rappers acting dramatic."
Timberlake's slam comes after West commented about the singer and Social Network actor at his concert at the Hammersmith Apollo.
West told the crowd: "I got love for Hov, but I ain't f-cking with that Suit & Tie."
His appearance on Saturday Night Live marked Timberlake's fifth time on the show.
He joined other 'Five Timers Club' members on stage to mark the event, including Steve Martin, Chevy Chase, Alec Baldwin, Candace Bergen and Tom Hanks.