League One Reading have been docked three points after their owner failed to deposit enough money into an account for the club's monthly wage bill.
Owner Dai Yongge was told by the EFL in August to put 125% of the wage bill into a designated account by Tuesday.
The EFL said he failed to meet the deadline which triggered the suspended points deduction with immediate effect.
The Royals, who have now been docked four points in total this season, drop to 21st in the table on two points.
Reading have said Chinese businessmen Dai remains "committed" to the Royals and is still actively looking for new investors to help provide financial stability at the club.
"Mr Dai is currently undertaking an ongoing process of seeking sources of stable external investment for the football club, with the aim of mitigating the risk of cashflow complications arising in future," a statement said on the club website.
The EFL, who docked the Royals a point in August for previous late wage payments, said it would continue to punish the Berkshire-based club if they failed to comply with the rules.
"The EFL continues to acknowledge the negative impact sporting sanctions are having on the football club," the league said in a statement.
"[We] remain extremely disappointed and frustrated at the club's ownership to meet its ongoing obligations under EFL Regulations.
"The League will continue to apply its rules in all circumstances deemed appropriate."
Reading also received a six-point deduction in April - for profit and sustainability rule breaches - that contributed to their relegation from the Championship.