Australia will face a weakened Indian side in the bulk of their 50-over World Cup warm-up series after the hosts opted to rest four key players from the first two matches.
They include captain Rohit Sharma so KL Rahul will lead India in those fixtures.
Also omitted are Virat Kohli, Hardik Pandya and Kuldeep Yadav.
India have already played six ODIs this September while winning the Asia Cup.
The squad for the third and final match is the one that will represent India in the World Cup on home soil - with the addition of Ravichandran Ashwin and Washington Sundar, who will be available for all three warm-ups.
Ashwin, India's preferred spinner in Test cricket, played the last of his 113 one-day internationals in January 2022 and was left out of the provisional squad announced earlier this month.
Axar Patel, who missed the Asia Cup final due to a quadriceps strain, has been named in the squad for the final ODI, but his participation is subject to fitness.
The three ODIs will be played between September 22-27 in Mohali, Indore and Rajkot. The World Cup begins on October 5 with Australia playing the hosts three days later in Chennai.
SQUAD FOR FIRST TWO ODIS
KL Rahul (capt), Ravindra Jadeja, Ruturaj Gaikwad, Shubman Gill, Shreyas Iyer, Suryakumar Yadav, Tilak Varma, Ishan Kishan , Shardul Thakur, Washington Sundar, R Ashwin, Jasprit Bumrah, Mohammed Shami, Mohammed Siraj, Prasidh Krishna
SQUAD FOR THIRD ODI
Rohit Sharma (capt), Hardik Pandya, Shubman Gill, Virat Kohli, Shreyas Iyer, Suryakumar Yadav, KL Rahul, Ishan Kishan, Ravindra Jadeja, Shardul Thakur, Axar Patel, Washington Sundar, Kuldeep Yadav, R Ashwin, Jasprit Bumrah, Mohammed Shami, Mohammed Siraj.